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Absence of FCS and exposed to ?BHRF1??ASO on proper concentratin could inhibit proliferation of SUNE?1.?
适当浓度BHRF1反义寡核苷酸片段能抑制无血清培养的SUNE?1细胞株的增殖。
Inhibited proliferation of SUNE?1 was observed in the absence of fetal calf serum(FCS) and exposed to ?BHRF1??ASO.
适当浓度BHRF1反义寡核苷酸片段能引起无血清培养的SUNE?1细胞株增殖受抑制；
Sune Keller,Francois Lauze, and Mads Nielsen, A Total Variation Motion Adaption Deinterlacing Scheme[J], The IT University of Copenhagen.
秦波，吴援明，杨健君，等，一种基于运动估计的隔行/逐行转换算法[J]，电科技大学学报，2002年，第31期，131-135。
Objective:To investigate the effects of EBV ?BHRF1? antisense oligo?deoxynucleotide (ASO) on the proliferation of poorly differentiated nasopharyngeal carcinoma (SUNE?1) cell lines.
目的：观察EB病毒BHRF1反义寡核苷酸片段对鼻咽癌SUNE?1细胞株增殖的影响。
Intact BHRF1, DNase, thymidine kinase (TK) and nuclear antigen?1 gene fragments of EBV were amplified using PCR technique with DNAs from B??95?8? and SUNE?1 cell lines as template.
PCR从SUNE?1细胞DNA中扩增出EB病毒的4种（BHRF1、DNA酶、胸苷激酶及核抗原?1）全基因片段；
Poorly differentiated nasopharyngeal carcinoma (SUNE 1) cell line was cultured with Epstein Barr virus (EBV) BHRF1, DNase, thymidine kinase (TK) and nuclear antigen 1 (EBNA1) antisense oligodeoxynucleotide (ASO) for 48 h respectively.
用不同浓度的EB病毒 4种 (BHRF1、DNA酶、核抗原 1及胸苷激酶 )反义寡核苷酸片段 ,(antisenseoligodeoxynucleotide ,ASO)分别与含 10%25胎牛血清或不含胎牛血清的人低分化鼻咽癌细胞株 (SUNE 1)共同培养 4 8h。

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